Expression of Multidrug Resistance Proteins and Accumulation of Cisplatin in Human Non-small Cell Lung Cancer Cells(Biopharmacy)
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概要
- 論文の詳細を見る
In order to understand and overcome multidrug resistance (MDR) of human non-small cell lung cancer (NSCLC), mRNA and protein expression levels of P-glycoprotein (MDR1), multidrug resistance-associated protein 1 (MRP1), and lung resistance-related protein (LRP) were investigated and compared with the chemosensitivity and the intracellular/intranuclear cisplatin accumulation of three NSCLC cell lines (Ma-10, Ma-31, and Ma-46). Ma-31 was more resistant than Ma-10 and Ma-46 to cisplatin, carboplatin, etoposide, and paclitaxel. The mRNA level of MDR1 was extremely low, and MDR1 protein was not detected in all cell lines. MRP1 mRNA expression was highest in Ma-31 and lowest in Ma-10, but there was no notable difference between the MRP1 protein expression in three cell lines. LRP mRNA/protein was equally expressed in Ma-10 and Ma-31, but was nominal in Ma-46. The intracellular/intranuclear cisplatin accumulation of the cells was determined to be Ma-31>Ma-46>Ma-10. Thus, MDR1, MRP1, and LRP mRNA and protein expression levels were not correlated with the chemosensitivity or the intracellular/intranuclear cisplatin accumulation of each cell line. The present results indicate that MDR proteins (MDR1, MRP1, and LRP) may not play an important role in the chemoresistance and drug efflux of NSCLC cells.
- 公益社団法人日本薬学会の論文
- 2005-04-01
著者
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NAITO Shinsaku
Research and Development Center, Otsuka Pharmaceutical Factory, Inc.
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Nishimura M
Research And Development Center Otsuka Pharmaceutical Factory Inc.
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Nishimura Masuhiro
Div. Of Pharmacology Drug Safety And Metabolism Otsuka Pharmaceutical Factory Inc.
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Nishimura Masuhiro
Department Of Drug Metabolism Division Of Pharmacology Drug Safety And Metabolism Otsuka Pharmaceuti
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Nishimura Masuhiro
Division Of Pharmacology Drug Safety And Metabolism Otsuka Pharmaceutical Factory Inc.
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UEDA Nobuhiko
Division of Pharmacology, Drug Safety and Metabolism, Otsuka Pharmaceutical Factory, Inc.
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NAITO Shinsaku
Division of Pharmacology, Drug Safety and Metabolism, Otsuka Pharmaceutical Factory, Inc.
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Shimizu Eiji
Division of Medical Oncology and Molecular Respirology, Faculty of Medicine, Tottori University, Jap
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Naito S
Division Of Applied Bioscience Graduate School Of Agriculture Hokkaido University
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山内 あい子
Department Of Pharmaceutical Information Science Graduate School Of Pharmaceutical Science The Unive
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Yamauchi Aiko
Graduate School Of Pharmaceutical Sciences The University Of Tokushima
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LEE Eibai
Department of Pharmacology, Faculty of Pharmaceutical Sciences
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Nishimura M
Division Of Experimental Animals Center For Promotion Of Medical Research And Education Graduate Sch
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IKUTA Kenji
Graduate School of Pharmaceutical Sciences, The University of Tokushima
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TAKEMURA Kazu
Graduate School of Pharmaceutical Sciences, The University of Tokushima
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SASAKI Kenichi
Graduate School of Pharmaceutical Sciences, The University of Tokushima
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KIHARA Masaru
Graduate School of Pharmaceutical Sciences, The University of Tokushima
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Naito Shinsaku
Research And Development Center Otsuka Pharmaceutical Factory Inc.
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Naito Shinsaku
Res. And Dev. Center Otsuka Pharmaceutical Factory Inc.
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Nishimura Masuhiro
Res. And Dev. Center Otsuka Pharmaceutical Factory Inc.
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Lee E
Department Of Environmental Engineering University Of Suwon
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Lee Eibai
Department Of Pharmacology Faculty Of Pharmaceutical Sciences
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Lee Eibai
Department Of Pharmacology Faculty Of Pharmaceutical Sciences Kobe Gakuin University
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Ikuta Kenji
Graduate School Of Pharmaceutical Sciences The University Of Tokushima
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Shimizu Eiji
Division Of Medical Oncology And Molecular Respirology Faculty Of Medicine Tottori University
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Yamauchi A
Graduate School Of Bioagricultural Science Nagoya University
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Kihara M
Graduate School Of Pharmaceutical Sciences The University Of Tokushima
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Kihara Masaru
Fac. Of Pharmaceutical Sciences The Univ. Of Tokushima
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Takemura Kazu
Graduate School Of Pharmaceutical Sciences The University Of Tokushima
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Ueda Nobuhiko
Division Of Pharmacology Drug Safety And Metabolism Otsuka Pharmaceutical Factory Inc.
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Naito S
Otsuka Pharmaceutical Factory Inc. Tokushima Jpn
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Naito Shinsaku
Division Of Pharmacology Drug Safety And Metabolism Otsuka Pharmaceutical Factory Inc.
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Ueda Nobuhiko
Division Of Pharmacology Drug Safety And Metabolism Otsuka Pharmaceutical Factory
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Shimizu E
Division Of Medical Oncology And Molecular Respirology Faculty Of Medicine Tottori University
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Sasaki Kenichi
Graduate School Of Pharmaceutical Sciences The University Of Tokushima
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Shimizu Eiji
Division Of Medical Oncology And Molecular Respirology Department Of Multidisciplinary Internal Medi
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Ueda N
Division of Pharmacology, Drug Safety and Metabolism, Otsuka Pharmaceutical Factory, Inc.
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