Enhancement of Myofibrillar Proteolysis Following Infusion of Amino Acid Mixture Correlates Positively with Elevation of Core Body Temperature in Rats
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概要
- 論文の詳細を見る
Administration of an amino acid (AA) mixture stimulates muscle protein synthesis and elevates core body temperature (T_b), as characteristically found under anesthetic conditions. We tested the hypothesis that not only AA given, but also AA produced by degradation of endogenous muscular protein are provided for muscle protein synthesis, which is further reflected in T_b modifications. Rats were intravenously administered an AA mixture or saline in combination with the anesthetic propofol or lipid emulsion. We measured plasma 3-methylhistidine (MeHis) concentrations as an index of myofibrillar protein degradation, rectal temperature and mRNA expression of atrogin-1, MuRF-1 and ubiquitin in gastrocnemius and soleus muscles of rats following 3h infusion of test solutions. T_b did not differ significantly between conscious groups, but was higher in the AA group than in the saline group among anesthetized rats. Plasma MeHis concentrations were higher in the AA group than in the saline group under both conditions. Plasma MeHis levels correlated positively with T_b of rats under both conditions. AA administration decreased mRNA levels of atrogin-1 and ubiquitin in gastrocnemius muscle and all mRNA levels in soleus muscle. These results suggest that AA administration enhances myofibrillar protein degradation and that the change is a determinant of T_b modification by AA administration. However, the mechanisms underlying AA administration-associated enhancement of myofibrillar proteolysis remains yet to be determined.
- 日本ビタミン学会の論文
著者
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Nishimura M
Research And Development Center Otsuka Pharmaceutical Factory Inc.
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Nishimura Masuhiro
Div. Of Pharmacology Drug Safety And Metabolism Otsuka Pharmaceutical Factory Inc.
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Nishimura Masuhiro
Department Of Drug Metabolism Division Of Pharmacology Drug Safety And Metabolism Otsuka Pharmaceuti
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Nishimura Masuhiro
Res. And Dev. Center Otsuka Pharmaceutical Factory Inc.
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Nakayama Mitsuo
Department Of Drug Metabolism Division Of Pharmacology Drug Safety And Metabolism Otsuka Pharmaceuti
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Kawano Yuichi
New Product And Business Development Research And Development Center Otsuka Pharmaceutical Factory I
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NISHIMURA Masuhiro
New Product and Business Development, Research and Development Center, Otsuka Pharmaceutical Factory
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MIKURA Mayumi
New Product and Business Development, Research and Development Center, Otsuka Pharmaceutical Factory
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NAKAYAMA Mitsuo
New Product and Business Development, Research and Development Center, Otsuka Pharmaceutical Factory
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YAMAOKA Ippei
New Product and Business Development Department, Research and Development Center, Otsuka Pharmaceuti
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DOI Masako
New Product and Business Development Department, Research and Development Center, Otsuka Pharmaceuti
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Doi Masako
Res. And Dev. Center New Business And Dev. Dep. Otsuka Pharmaceutical Factory Inc.
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Yamaoka Ippei
Res. And Dev. Center New Business And Dev. Dep. Otsuka Pharmaceutical Factory Inc.
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Yamaoka Ippei
New Product And Business Development Department Research And Development Center Otsuka Pharmaceutica
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Doi Masako
New Product And Business Development Department Research And Development Center Otsuka Pharmaceutica
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Mikura Mayumi
New Product And Business Development Research And Development Center Otsuka Pharmaceutical Factory I
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