資料 Tissue Distribution of mRNA Expression of Human Cytochrome P450 Isoforms Assessed by High-Sensitivity Real-Time Reverse Transcription PCR
スポンサーリンク
概要
- 論文の詳細を見る
Pairs of forward and reverse primers and TaqMan probes specific to each human cytochrome P450 isoform were prepared. Analysis of the mRNA level of each CYP isoform in total RNA from pooled specimens of various human organs was performed by real-time reverse transcription PCR using an ABI PRISM 7700 sequence detector system. The expression of CYP3A4 mRNA was similar to that of CYP3A7 mRNA in the fetal liver, and CYP3A4 mRNA levels in the fetal liver were about 0.1 times lower than in the adult liver. CYP2E1 showed the highest level of mRNA expression in the liver. The mRNA expression of 30 CYP isoforms (CYP1A1, 1A2, 1B1, 2A6, 2A7, 2B6, 2C8, 2C9, 2C18, 2C19, 2D6, 2E1.2J2, 3A4, 3A5, 3A7, 4A11.4F2, 4F3, 5A1, 7B1, 8A1, 8B1, 17, 26A1, 27, 27B1, 39A1, 46, and 51) in the liver was successfully detected by this method. CYP2F1, 4B1, 4F8, 11s (11 A, 11B1, and 11B2), 19, and 24 mRNA levels were the highest in the lung, lung, prostate, adrenal gland, placenta, and kidney, respectively; however, the mRNA expression of these eight CYP isoforms in the liver was not detected by this method. The mRNA levels of the CYP isoforms determined in various human tissues were in good agreement with previously reported data. The method described here has the advantages of high specificity and excellent quantification over a wide range of mRNA concentrations, making it suitable for the evaluation of a large number of samples in the assessment of the expression profile of drug-metabolizing enzymes.
- 公益社団法人日本薬学会の論文
- 2003-05-01
著者
-
NAITO Shinsaku
Research and Development Center, Otsuka Pharmaceutical Factory, Inc.
-
Nishimura M
Research And Development Center Otsuka Pharmaceutical Factory Inc.
-
Nishimura Masuhiro
Department Of Drug Metabolism Division Of Pharmacology Drug Safety And Metabolism Otsuka Pharmaceuti
-
NAITO Shinsaku
Department of Drug Metabolism, Division of Pharmacology, Drug Safety and Metabolism, Otsuka Pharmace
-
YAGUTI Hiroshi
Department of Drug Matabolism Division of Pharmacology,Drug Safety and metabolism,Otsuka Pharmaceuti
-
YOSHITSUGU Hiroki
Department of Drug Matabolism Division of Pharmacology,Drug Safety and metabolism,Otsuka Pharmaceuti
-
SATOH Tetsuo
Non^Profit Organization Human Anima Bridging Research Organization
-
Satoh Tetsuo
Non-profit Organization Human & Animal Bridging Research Institute Ichikawa General Hospital
-
Yaguti Hiroshi
Department Of Drug Matabolism Division Of Pharmacology Drug Safety And Metabolism Otsuka Pharmaceuti
-
Naito S
Otsuka Pharmaceutical Factory Inc. Tokushima Jpn
-
Yoshitsugu Hiroki
Division Of Pharmacology Drug Safety And Metabolism Otsuka Pharmaceutical Factory Inc.
-
西村 益浩
Department of Drug Matabolism Division of Pharmacology,Drug Safety and metabolism,Otsuka Pharmaceutical Factory,Inc.
-
矢口 寛
Department of Drug Matabolism Division of Pharmacology,Drug Safety and metabolism,Otsuka Pharmaceutical Factory,Inc.
-
吉次 博紀
Department of Drug Matabolism Division of Pharmacology,Drug Safety and metabolism,Otsuka Pharmaceutical Factory,Inc.
-
内藤 真策
Department of Drug Matabolism Division of Pharmacology,Drug Safety and metabolism,Otsuka Pharmaceutical Factory,Inc.
-
佐藤 哲男
Non^Profit Organization Human Anima Bridging Research Organization
関連論文
- Pharmacological assessment of ARTCEREB_【○!R】 irrigation and perfusion solution for cerebrospinal surgery using primary cultures of rat brain cells
- Different Effects of Desipramine on Bufuralol 1"-Hydroxylation by Rat and Human CYP2D Enzymes(Pharmacology)
- Comparative analysis of ATP-binding cassette (ABC) transporter gene expression levels in peripheral blood leukocytes and in liver with hepatocellular carcinoma
- Cytochrome P450 gene expression levels in peripheral blood mononuclear cells in comparison with the liver
- Perfusion fluids used in neurosurgery affect cerebrospinal fluid and surrounding brain parenchyma in the rat ventriculocisternal perfusion model
- Effects of NO-1886 (Ibrolipim), a Lipoprotein Lipase-Promoting Agent, on Gene Induction of Cytochrome P450s, Carboxylesterases, and Sulfotransferases in Primary Cultures of Human Hepatocytes
- Influence of Albumin on Enantioselective Local Disposition of BOF-4272,a Xanthine Oxidase Inhibitor with Chiral Sulfoxide, in Rat Liver
- Hepatic Local Disposition of a Drug with High Protein Binding and High Hepatic Clearance Using BOF-4272 as a Model Drug
- カニクイザルにおける薬物代謝酵素およびトランスポーターmRNAの組織発現プロファイル
- ヒト組織におけるトランスポーター-SLC35ファミリーのmRNA発現プロファイル
- Comparison of Inducibility of Multidrug Resistance (MDR)1, Multidrug Resistance-Associated Protein (MRP)1, and MRP2 mRNAs by Prototypical Microsomal Enzyme Inducers in Primary Cultures of Human and Cynomolgus Monkey Hepatocytes(Pharmacology)
- Comparison of Inducibility of Sulfotransferase and UDP-Glucuronosyltransferase mRNAs by Prototypical Microsomal Enzyme Inducers in Primary Cultures of Human and Cynomolgus Monkey Hepatocytes
- One-Point サンプリングデータのブートストラップ法による薬物速度論パラメータのヒストグラム解析
- Soybean Oil Fat Emulsion to Prevent TPN-Induced Liver Damage : Possible Molecular Mechanisms and Clinical Implications
- Assessment of Induction of Cytochrome P450 by NO-1886 (Ibrolipim), a Lipoprotein Lipase-Promoting Agent, in Primary Cultures of Human Hepatocytes and in Female Rat Liver
- Major Role of Soybean Oil in Preventing Decrease in CYP4A1 mRNA Content Relative to PPARα mRNA Down-Regulation in Infant Rats(Pharmacology)
- Soybean Oil in Total Parenteral Nutrition Maintains Albumin and Antioxidant Enzyme mRNA Levels(Biopharmacy)
- Expression of Multidrug Resistance Proteins and Accumulation of Cisplatin in Human Non-small Cell Lung Cancer Cells(Biopharmacy)
- Role of Soybean Oil Fat Emulsion in the Prevention of Hepatic Xenobiotic Transporter mRNA Up- and Down-Regulation Induced by Overdose of Fat-free Total Parenteral Nutrition in Infant Rats
- Soybean Oil Fat Emulsion Prevents Cytochrome P450 mRNA Down-Regulation Induced by Fat-Free Overdose Total Parenteral Nutrition in Infant Rats(Biopharmacy)
- Effects of Pretreatment of Hep G2 Cells with β-Naphthoflavone on Cytotoxicity of Propranolol and its Active Metabolite 4-Hydroxypropranolol
- Effects of Dimethyl Sulfoxide on the Gene Induction of Cytochrome P450 Isoforms, UGT-Dependent Glucuronosyl Transferase Isoforms, and ABCB1 in Primary Culture of Human Hepatocytes
- 資料 Tissue Distribution of mRNA Expression of Human Cytochrome P450 Isoforms Assessed by High-Sensitivity Real-Time Reverse Transcription PCR
- Effects of Dimethyl Sulphoxide and Dexamethasone on mRNA Expression of Myogenesis- and Muscle Proteolytic System-related Genes in Mouse Myoblastic C2C12 Cells
- Intrathecal Disposition of ARTCEREB® Irrigation and Perfusion Solution for Cerebrospinal Surgery in Rats
- ヒトの肝細胞培養系を用いた新薬候補化合物曝露のCYP1A2およびCYP3A4誘導能の評価
- Enhancement of Myofibrillar Proteolysis Following Infusion of Amino Acid Mixture Correlates Positively with Elevation of Core Body Temperature in Rats
- Analysis of gene and protein expression of cytochrome P450 and stress-associated molecules in rat liver after spaceflight
- Secretion of Albumin and Induction of CYP1A2 and CYP3A4 in Novel Three-dimensional Culture System for Human Hepatocytes using Micro-space Plate
- Induction of Human CYP1A2 and CYP3A4 in Primary Culture of Hepatocytes from Chimeric Mice with Humanized Liver
- Evaluation of Human CYP1A2 and CYP3A4 mRNA Expression in Hepatocytes from Chimeric Mice with Humanized Liver
- Tissue-specific mRNA Expression Profiles of Human Nuclear Receptor Subfamilies
- Metabolic Pathways and Pharmacokinetics of BOF-4272,a Sulfoxide-Containing Drug, in the Dog : In Vivo and in Vitro Studies
- Morphological Changes on the Free Surface of Thyroid Cells at the Hormone Secretion by Scanning Electron Microscopy