Morphological Changes on the Free Surface of Thyroid Cells at the Hormone Secretion by Scanning Electron Microscopy

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Early effects of thyrotropin (TSH) in vivo and in vitro on the apical surface of thyroid follicular cells of rats pretreated with thyroxine were observed with a scanning electron microscope by applying our improved technical method. Numerous large pseudopods appeared and also in some cases apertures were observed at the apices of these pseudopods after the short interval after TSH stimulation in vivo. The effect of TSH in vitro was essentially the same as that in vivo. The formation of pseudopods was correspondingly stimulated in vivo by the administration of propylthiouracil (PTU) within the short interval (1.5 hr). The pattern of pseudopod formation by PTU was different from that observed in TSH stimulation. The early effect of PTU coincides with our previous biochemical result that thiouracil in vivo might acutely stimulate the activity of engulfment of intrafollicular colloid. It was demonstrated by the transmission electron microscope that the polystyrene beads, 0.25μm in diameter, could enter the cells presumably through the aperture opened at the appex of the pseudopod when they were placed in the incubation medium containing TSH. This fact suggests that the pseudopods play an important role in the endocytic process regardless of the nature of engulfed materials. Despite the diurnal changes of thyroid activity in the normal rats, no cyclic changes of pseudopods were observed with the scanning electron microscope. The normal pattern of colloid resorption under physiological conditioni was discussed through the comparison of TSH concentration with the morphological changes of pseudopods.
社団法人日本内分泌学会の論文

Morphological Changes on the Free Surface of Thyroid Cells at the Hormone Secretion by Scanning Electron Microscopy

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