Resting spore of plasmodiophora brassicae proliferates only in the callus of clubroot disease-susceptible turnip but increases the PAL activity in the callus of clubroot disease-resistant turnip
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概要
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The calluses were indeced from turnips, Brassicae campestris L. which were susceptible and resistant to Plasmodiophora brassicae in Murashige-Skoog's agar medium supplemented with 1.0 ppm 6-benzylaminopurine and 0.5 ppm α-naphthalene acetic acid. When a 10 μl water suspension containing 104 resting spores of P. brassicae was placed on the surface of the calluses, about 3 × 105 zoosporangium-like spheroids (SLS) were recovered from the susceptible calluses after 24 h of the treatment but no SLS was found in the resistant callus. On 6th day after the treatment, the SLS increased to about 4 × 106 in the susceptible callus. Upon inoculation of resistant callus with 104 resting spores, the phenylalanine ammonia lyase (PAL) activity increased about 4-fold after 20 h, however, such increase was not observed in the susceptible callus during the same period. The consitutive PAL activity of susceptible callus was roughly one 6th of that of resistant callus.
- 日本植物細胞分子生物学会の論文
- 2001-12-01
著者
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Itoh Kimiko
Graduate School Of Sci. And Technol. Niigata Univ.
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Hori Hidetaka
Graduate School of Science and Technology, Niigata University
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Mitsui Toshiaki
Graduate School of Science and Technology, Niigata University
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Hori H
Graduate School Of Sci. And Technol. Niigata Univ.
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Hori Hidetaka
Laboratories Of Molecular Life Sciences Graduate School Of Natural Science And Technology Niigata Un
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Hori Hidetaka
Graduate School Of Natural Science And Technology Niigata University
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Mitsui Toshiaki
Graduate School Of Natural Science And Technology Niigata University
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Muraoka Satoko
Department Of Agriculture Niigata University
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ITO Kimiko
Department of Agriculture, Niigata University
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KISO Akira
Musashino Shubyo En Corp.
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Takahashi Hideyuki
Graduate School Of Agricultural And Life Sciences The University Of Tokyo
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Hori Hidetaka
Laboratories Of Plant And Mircobial Genome Control Graduate School Of Science And Technology Niigata
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Takahashi Hideyuki
Graduate School Of Science And Technology Niigata University
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