Fractionation and Some Features of Synergistic Compound Which Enhances the Insecticidal Activity of δ-Endotoxin of Bacillus thuringiensis

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概要

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• The supernatant of Bacillus thuringiensis culture has been shown to substantially enhance the larvicidal activity of the δ-endotoxin against Spodoptera litura, indicating the existence of synergistic activity in the supernatant (ASANO et al. (1994) Appl. Entomol. Zool. 29: 39-45; ASANO and HORI (1995) Appl. Entomol. Zool. 30: 369-374). The factor responsible for this synergistic activity was fractionated from culture supernatant using cation exchange and gel filtration chromatography. The molecular weight was estimated to be about 400 by gel filtration. The factor was adsorbed on carboxymethy (CM) Sepharose equilibrated with 50 m_N Tris HCl buffer, pH 8.0 and eluted by the same buffer containing 0.2 M NaCl. Thus, the factor was apparently positively charged. Neigher α-, β-glucosidase nor pronase treatments caused a reduction in the synergistic activity. The fractionated synergistic factor significantly enhanced the activity of ICP against S. litura and S. exigua but not that against Plutella xylostella, Bombyx mori or Adoxophyes sp.

• 日本応用動物昆虫学会の論文
• 1996-02-25

著者

• Hori Hidetaka

  Graduate School of Science and Technology, Niigata University

• Hori H

  Graduate School Of Sci. And Technol. Niigata Univ.

• Hori Hidetaka

  Laboratories Of Molecular Life Sciences Graduate School Of Natural Science And Technology Niigata Un

• Hori Hidetaka

  Graduate School Of Natural Science And Technology Niigata University

• Hori Hidetaka

  Biopesticide Laboratory At The Advanced Technology Institute Kubota Corporation

• Asano Shoji

  Biopesticide Laboratory And The Advanced Technology Institute Kubota Corporation

• Asano Shoji

  Advanced Technology Laboratory Kubota Corp.

• Hori Hidetaka

  Biopesticide Laboratories Advanced Technology Institute Kubota Corporation

• OGIWARA Katsutoshi

  Biopesticide Laboratory and the Advanced Technology Institute, Kubota Corporation

• Ogiwara K

  Advanced Technology Laboratory Kubota Corp.

• Asano Shoji

  Biopesticide Laboratories Advanced Technology Institute Kubota Corporation

• Hori Hidetaka

  Laboratories Of Plant And Mircobial Genome Control Graduate School Of Science And Technology Niigata

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