Proteomic Identification of α-Amylase Isoforms Encoded by RAmy3B/3C from Germinating Rice Seeds

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概要

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•   We isolated and identified 10 α-amylase isoforms by using β-cyclodextrin Sepharose affinity column chromatography and two-dimensional polyacrylamide gel electrophoresis from germinating rice (Oryza sativa L.) seeds. Immunoblots with anti-α-amylase I-1 and II-4 antibodies indicated that 8 isoforms in 10 are distinguishable from α-amylase I-1 and II-4. Peptide mass fingerprinting analysis showed that there exist novel isoforms encoded by RAmy3B and RAmy3C genes. The optimum temperature for enzyme reaction of the RAmy3B and RAmy3C coding isoforms resembled that of α-amylase isoform II-4 (RAmy3D). Furthermore, complex protein polymorphism resulted from a single α-amylase gene was found to occur not only in RAmy3D, but also in RAmy3B.

• 社団法人 日本農芸化学会の論文
• 2004-01-23

著者

• Itoh Kimiko

  Graduate School Of Sci. And Technol. Niigata Univ.

• Hori Hidetaka

  Graduate School of Science and Technology, Niigata University

• Mitsui Toshiaki

  Graduate School of Science and Technology, Niigata University

• Hori H

  Graduate School Of Sci. And Technol. Niigata Univ.

• Hori Hidetaka

  Laboratories Of Molecular Life Sciences Graduate School Of Natural Science And Technology Niigata Un

• Hori Hidetaka

  Graduate School Of Natural Science And Technology Niigata University

• Nanjo Yohei

  Graduate School Of Sci. And Technol. Niigata Univ.

• ASATSUMA Satoru

  Graduate School of Science and Technology, Niigata University

• Mitsui T

  Graduate School Of Science And Technology Niigata University

• Mitsui Toshiaki

  Laboratories Of Molecular Life Science Graduate School Of Science And Technology Niigata University

• Mitsui Toshiaki

  Graduate School Of Natural Science And Technology Niigata University

• Asatsuma Satoru

  Graduate School Of Science And Technology Niigata University

• Mitsui Toshiaki

  Laboratories Of Plant And Mircobial Genome Control Graduate School Of Science And Technology Niigata

• Hori Hidetaka

  Laboratories Of Plant And Mircobial Genome Control Graduate School Of Science And Technology Niigata

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