Dissection of gene function by RNA silencing

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概要

• 論文の詳細を見る

• The RNA silencing technique is an effective tool to examine the biological function of the target mRNA in plants. The recent development of versatile-type RNAi vectors, which are driven by constitutive promoters, and GATEWAY™ cloning technology makes it easy to construct the RNAi vectors with trigger sequences and to analyze the function of a target gene. Although these vectors are highly useful, constitutive defects of the target mRNA expression sometimes result in lethality or seed abortion. Here, we summarize recent approaches to RNA silencing research designed to overcome these difficulties and to dissect gene expression.

• 日本植物細胞分子生物学会の論文
• 2005-12-01

著者

• Itoh Kimiko

  Graduate School Of Sci. And Technol. Niigata Univ.

• Itoh Kimiko

  Graduate School Of Science And Technology Niigata University

• ISLAM S.

  Graduate School of Science and Technology, Niigata University

• MIYAZAKI Takahiro

  Graduate School of Science and Technology, Niigata University

• TANNO Fuminori

  Center for Transdisciplinary Research, Niigata University

• Tanno Fuminori

  Center For Transdisciplinary Research Niigata University

• Islam S.

  Graduate School Of Science And Technology Niigata University

• Miyazaki Takahiro

  Graduate School Of Science And Technology Niigata University

関連論文

• Differential localizations and functions of rice nucleotide pyrophosphatase/phosphodiesterase isozymes 1 and 3
• Phosphate Modulates Ca^ Uptake and α- Amylase Secretion in Suspension - Cultured Cells of Rice
• Possible Involvement of Phosphoinositide-Ca^ Signaling in the Regulation of α-Amylase Expression and Germination of Rice Seed(Oryza sativa L.)
• Proteomic Identification of α-Amylase Isoforms Encoded by RAmy3B/3C from Germinating Rice Seeds
• Effect of Osmotic Stress on Turgor Pressure in Mung Bean Root Cells
• Role of K^+ and Cl^- in Osmotic Adjustment in Roots and Hypocotyls of Intact Mung Bean Seedlings
• Characterization of Inositol 1,4,5-Trisphosphate-Receptor Like Glycoprotein in Rice Cells
• Involvement of α-Amylase I-1 in Starch Degradation in Rice Chloroplasts
• Resting Spore of Plasmodiophora brassicae Proliferates Only in the Callus of Clubroot Disease-Susceptible Turnip but Increases the PAL Activity in the Callus of Clubroot Disease-Resistant Turnip
• Sucrose-Controlled Transport and Turnover of α-Amylase in Rice (Oryza sativa L.) Cells
• Proteomic Characterization of Tissue Expansion of Rice Scutellum Stimulated by Abscisic Acid
• Osmotic Adjustment and Osmotic Constituents in Roots of Mung Bean Seedlings
• Dissection of gene function by RNA silencing
• Differential localizations and functions of rice nucleotide pyrophosphatase/phosphodiesterase isozymes 1 and 3
• Phosphate modulates Ca2+ uptake and α-amylase secretion in suspension-cultured cells of rice
• Resting spore of plasmodiophora brassicae proliferates only in the callus of clubroot disease-susceptible turnip but increases the PAL activity in the callus of clubroot disease-resistant turnip

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