Phosphate Modulates Ca^<2+> Uptake and α- Amylase Secretion in Suspension - Cultured Cells of Rice

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概要

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• Effects of phosphate on the Ca2+ uptake and the sucrose-controlled secretion of α-amylase molecules in cultured rice cells were investigated. Phosphate markedly stimulated Ca2+ uptake into rice cells, particularly at the outer cell layer of the cell cluster. Phosphate also increased the synthesis and extracellular liberation of α-amylase II-4 in sucrose-supplemented cells. The distribution pattern of enzyme in rice cell clusters induced by phosphate was similar to that of Ca2+ uptake. Phosphate did not increase the level of mRNA of α-amylase II-4, indicating that phosphate stimulates the translation and posttranslational secretory processes of α-amylase II-4 in the presence of sucrose. Furthermore, phosphate enhanced both the Ca2+ uptake and α-amylase II-4 synthesis in the microsomes. These results strongly suggested that the ratio of phosphate to sugar is important for regulating the Ca2+ uptake, and that phosphate and sugar precisely coordinate the Ca2+-mediated synthesis and extracellular liberation of α-amylase II-4.

• 日本植物細胞分子生物学会の論文
• 2003-09-01

著者

• Kashem Mohammad

  Graduate School Of Science And Technology Niigata University

• Itoh Kimiko

  Graduate School Of Sci. And Technol. Niigata Univ.

• Hori Hidetaka

  Graduate School of Science and Technology, Niigata University

• Mitsui Toshiaki

  Graduate School of Science and Technology, Niigata University

• Hori H

  Graduate School Of Sci. And Technol. Niigata Univ.

• Hori Hidetaka

  Graduate School Of Natural Science And Technology Niigata University

• Nanjo Yohei

  Graduate School Of Sci. And Technol. Niigata Univ.

• ASATSUMA Satoru

  Graduate School of Science and Technology, Niigata University

• OHSHIMA Masahiro

  Graduate School of Science and Technology, Niigata University

• Mitsui T

  Graduate School Of Science And Technology Niigata University

• Mitsui Toshiaki

  Laboratories Of Molecular Life Science Graduate School Of Science And Technology Niigata University

• Mitsui Toshiaki

  Graduate School Of Natural Science And Technology Niigata University

• Ohshima Masahiro

  Graduate School Of Science And Technology Niigata University

• Asatsuma Satoru

  Graduate School Of Science And Technology Niigata University

• Hori Hidetaka

  Laboratories Of Plant And Mircobial Genome Control Graduate School Of Science And Technology Niigata

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