Purification and Characterization of Golgi Membrane-Bound Nucleoside Diphosphatase from Suspension-Cultured Cells of Sycamore (Acer pseudoplatanus L. )
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概要
- 論文の詳細を見る
Inosine diphosphatase (IDPase) isoforms associated with Golgi membranes were studied in sycamore cell culture. These enzyme isoforms were solubilized with Triton X-100 and purified by chromatography using DEAE-Toyopearl and SOURCE-S columns. The isoforms were separated into two distinguishable fractions (peak 1 and 2) by SOURCE-S column chromatography. Furthermore the peak 1 contained at least two isoform bands detected by native-PAGE analysis. The apparent molecular sizes of these three isoforms were estimated by both gel filtration and SDS-PAGE to be 50 kDa, indicating that the Golgi membrane-bound IDPase has a monomeric structure. These IDPase isoforms required divalent cations (Ca2+, Mg2+, Co2+, Mn2+) for their hydrolyzing activity, and were inhibited by ATP. IDP, UDP, and GDP were effective substrates for these enzymes. It is clearly indicated that the sycamore Golgi membrane-bound IDPase is a nucleoside diphosphatase.
- 日本植物細胞分子生物学会の論文
- 2001-12-01
著者
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Mikami Satoshi
Laboratories Of Molecular Life Science Graduate School Of Science And Technology Niigata University
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Mikami S
Niigata Univ. Niigata Jpn
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Hori Hidetaka
Laboratories Of Molecular Life Science Graduate School Of Science And Technology Niigata University
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Mitsui Toshiaki
Laboratories of Molecular Life Sciences, Graduate School of Science and Technology, Niigata Universi
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Mitsui T
Graduate School Of Science And Technology Niigata University
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Mitsui Toshiaki
Laboratories Of Molecular Life Science Graduate School Of Science And Technology Niigata University
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SUGANUMA Ryo
Laboratories of Molecular Life Science, Graduate School of Science and Technology, Niigata Universit
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Suganuma Ryo
Laboratories Of Molecular Life Science Graduate School Of Science And Technology Niigata University
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Mitsui T
Laboratories Of Molecular Life Science Graduate School Of Science And Technology Niigata University
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Mitsui T
Graduate School Of Natural Science And Technology Niigata University
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