Characterization of ATPase Activity in Brush Border Membrane Vesicles from the Silkworm, Bombyx mori(Biological Chemistry)
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概要
- 論文の詳細を見る
Brush border membrane vesicles (BBMV) from the midgut epithelial cells of silkworm larvae were prepared. ATP hydrolyzing activity (ATPase activity) was associated with the BBMV. ATPase activity without Mg^<2+> was not observed at pH 7 but substantial ATP hydrolyzing activity was observed at pH 7 with Mg^<2+>. The enzyme required Mn^<2+>, Mg^<2+>, or Ca^<2+> ions. The enzyme also hydrolyzed ITP and GTP but not p-NPP, ADP, or AMP. KNO_3 and NEM strongly inhibited the ATPase activity. Behaviours of the ATPase against inhibitors suggested that it resembled vacuolar type ATPase.
- 社団法人日本農芸化学会の論文
- 1991-11-23
著者
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Hori Hidetaka
Laboratories Of Molecular Life Science Graduate School Of Science And Technology Niigata University
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Hori Hidetaka
Laboratory Of Biochemistry And Genetic Engineering Tsukuba Laboratories Of Research And Development
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Minami Masayoshi
Laboratory Of Biochemistry And Genetic Engineering Tsukuba Laboratories Of Research And Development
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Indrasith Leslie
Laboratory Of Biochemistry And Genetic Engineering Tsukuba Laboratories Of Research And Development
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