Technical Improvement to 2D-PAGE of Rice Organelle Membrane Proteins(Analytical Chemistry)
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概要
- 論文の詳細を見る
Cytosolic and membrane-associated proteins prepared from rice cells were separated and compared by two different 2D-PAGE methods, isoelectric focusing (IEF)/SDS-PAGE and nonequilibrium pH gradient electrophoresis (NEPHGE)/SDS-PAGE. Although IEF/SDS-PAGE of the cytosolic proteins showed sufficient resolution, some mitochondrial and basic microsomal membrane-associated proteins were weakly or hardly detectable on the 2D gel. High-quality and -quantity separation of the organelle membrane-associated proteins was accomplished by NEPHGE/SDS-PAGE, the advantage of this method being more critical in tightly membrane-bound proteins that were unwashable with NaCl. These results indicate that NEPHGE/SDS-PAGE is a useful tool for the proteomic analysis of rice membrane-associated proteins.
- 社団法人日本農芸化学会の論文
- 2002-05-23
著者
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Mikami Satoshi
Laboratories Of Molecular Life Science Graduate School Of Science And Technology Niigata University
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Mikami S
Niigata Univ. Niigata Jpn
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Mitsui Toshiaki
Graduate School of Science and Technology, Niigata University
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Hori Hidetaka
Laboratories Of Molecular Life Science Graduate School Of Science And Technology Niigata University
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Mitsui Toshiaki
Laboratories of Molecular Life Sciences, Graduate School of Science and Technology, Niigata Universi
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Mitsui T
Graduate School Of Science And Technology Niigata University
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Mitsui Toshiaki
Laboratories Of Molecular Life Science Graduate School Of Science And Technology Niigata University
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KISHIMOTO Tadashi
Laboratories of Molecular Life Science, Graduate School of Science and Technology, Niigata Universit
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Mitsui T
Laboratories Of Molecular Life Science Graduate School Of Science And Technology Niigata University
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Mitsui T
Graduate School Of Natural Science And Technology Niigata University
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