Extracellular Matrix Patterning for Cell Alignment by Atmospheric Pressure Plasma Jets
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概要
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Low-temperature atmospheric-pressure plasma (APP) jets and a metal stencil mask have been used for the patterning of fibronectins deposited on a silicon (Si) wafer. Fibronectins typically constitute the extracellular matrix (ECM) and a micro-patterned ECM may be used for arranging living cells in a desired pattern on the substrate surface. Such a technique can be used for the fabrication of cell chips. In this study, patterning of 100-μm-wide lines of fibronectin layers has been demonstrated. Desorption of fibronectins from the surface by plasma application has been confirmed by atomic force microscopy (AFM) and Fourier transform infrared spectroscopy (FT-IR).
- 2012-03-25
著者
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Hamaguchi Satoshi
Center For Atomic And Molecular Technologies Graduate School Of Engineering Osaka University
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Kitano Katsuhisa
Center For Atomic And Molecular Technologies Osaka Univ.
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Hamaguchi Satoshi
Center for Atomic and Molecular Technologies, Osaka University, Suita, Osaka 565-0871, Japan
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Sayed Md.
Department of Structural Molecular Science, The Graduate University for Advanced Studies, Okazaki, Aichi 444-8585, Japan
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Ando Ayumi
Center for Atomic and Molecular Technologies, Osaka University, Suita, Osaka 565-0871, Japan
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Asano Toshifumi
Division of Neuroscience, Department of Developmental Biology and Neurosciences, Tohoku University, Sendai 980-8577, Japan
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Tero Ryugo
Electronics-Inspired Interdisciplinary Research Institute, Toyohashi University of Technology, Toyohashi, Aichi 441-8580, Japan
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Urisu Tsuneo
FIRST Research Center for Innovative Nanobiodevice, Nagoya University, Nagoya 464-8603, Japan
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Kitano Katsuhisa
Center for Atomic and Molecular Technologies, Osaka University, Suita, Osaka 565-0871, Japan
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URISU Tsuneo
FIRST Research Center for Innovative Nanobiodevice, Nagoya University, Furo-cho, Chikusa, Nagoya, Aichi 464-8603, Japan
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ASANO Toshifumi
Division of Neuroscience, Department of Developmental Biology and Neurosciences, Tohoku University, 2-1-1 Katahira, Aoba, Sendai, Miyagi 980-8577, Japan
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ANDO Ayumi
Center for Atomic and Molecular Technologies, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan
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