Surface Modification of Gel-Free Microchannel Surface Electrophoresis Device for DNA Identification
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概要
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A gel-free microchannel electrophoresis device for DNA separation and identification was studied. DNA fragments ranging from 3.5 to 21.2 kbp were effectively separated and identified. The channel’s bottom surface was composed of silicon wafer or glass, and the channel wall was composed of SU-8 photoresist. The channel surface was modified with various solutions or plasmas. The separated DNA on the detection electrode was confirmed by electron spectroscopy for chemical analysis. DNA mobility on the glass substrate was higher than that on the Si substrate. In addition, the increase of the ionic strength of the solution on the device decreased the mobility of DNA. In summary, this new device is applicable to large DNA samples.
- 2008-04-25
著者
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Kuo Yue
Thin Film Microelectronics Research Laboratory Department Of Chemical Engineering Texas A&m Univ
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Lee Hyun
Department Of Anesthesia And Pain Medicine School Of Medicine Pusan National University
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Kuo Yue
Thin Film Nano and Microelectronics Research Laboratory, Department of Chemical Engineering, Texas A&M University, TX 77843-3122, U.S.A.
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Lee Hyun
Department of Chemical Engineering, Myongji University, Yongin 449-728, Republic of Korea
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