Second-Harmonic-Generation Microscopy Using Excitation Beam with Controlled Polarization Pattern to Determine Three-Dimensional Molecular Orientation
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概要
- 論文の詳細を見る
We have developed a second-harmonic-generation (SHG) microscope using an excitation beam with a controlled polarization pattern in order to detect three-dimensional molecular orientation. The electric field at the focus is controlled three-dimensionally by modifying the polarization distribution with a parallel-aligned nematic-liquid-crystal spatial-light-modulator without any mechanical moving parts. We demonstrated that the SHG signal from an Achilles tendon, sliced so that collagen fibers were aligned parallel to the optical axis, excited by a radially polarized beam was higher than those excited by linearly polarized beams. The possibility of determinating three-dimensional molecular orientation was thus shown.
- Japan Society of Applied Physicsの論文
- 2005-08-10
著者
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Araki Tsutomu
Department Of Applied Physics Faculty Of Engineering Osaka University
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Hashimoto Mamoru
Department Of Applied Chemistry Faculty Of Engineering Kyushu Institute Of Technology
-
Hashimoto Mamoru
Department of Mechanical Science and Bioengineering, Graduate School of Engineering Science, Osaka University, Machikaneyama 1-3, Toyonaka, Osaka 560-8531, Japan
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Yoshiki Keisuke
Department of Mechanical Science and Bioengineering, Graduate School of Engineering Science, Osaka University, Machikaneyama 1-3, Toyonaka, Osaka 560-8531, Japan
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