Enzymatic Method for Determination of Inulin

概要

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A new, totally enzymatic method for the determination of inulin in serum and urine, using inulase (EC 3.2.1.7.), fructokinase (EC 2.7.1.4), phosphoglucoisomerase (EC 5. 3. 1. 9), and glucose- 6- phosphate dehydrogenase (EC 1. 1. 1.49) is described. The assay is carried out by means of a fructose determination after hydrolysis of inulin with inulase. The calibration curve was linear up to 2g/l of inulin. The within- run coefficients of variation were found to be between 1.1 and 2.3%. Data showing the consistent quantitative recovery of added inulin from serum and urine are presented. Thirty-seven serum-urine samples for renal glomerular filtration rate were used to obtain correlations between this method (Y) and the thiosulfate method (X). The correlation coefficients and regression equations were as follows r=0.9785, Y=0.8902X-0.92. This method is simpler and faster than those used in the past, and has shown itself to be practical for use in clinical laboratories.
Japan Society of Clinical Chemistryの論文