Inhibition of .BETA.-CCM of cholecystokinin-induced release of acetylcholine from the longitudinal muscle-myenteric plexus preparation in the guinea-pig ileum.

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Itonaga, M., Sunagane, N., Uruno, T. and Kubota, K. Inhibition by β-CCM of cholecys-tokinin-induced release of acetylcholine from the longitudinal muscle-myenteric plexus preparation in the guinea-pig ileum. Japanene Journal of Smooth Muscle Research, 26: 1-7, 1990. The antagonism between cholecystokinin (CCK) and methyl β-carboline-3-carbox-ylate (β-CCM) in the nervous system was studied by measuring the release of acetylcholine (ACh) from the longitudinal muscle-myenteric plexus preparation of guinea-pig. The ACh release was assessed by measuring [3H] output from the preparation preincubated with [3H] choline. Thirty mM of KCl caused a pronounced relase of [3H] ACh from the preparation. Sulfated cholecystokinin octapeptide (CCK8) also increased the release of [3H] ACh in a dose-dependent manner at concentrations ranging from 10-10M to 10-8M. CCK8 at a concentration of 10-8M released [3H] ACh by about 300% of the 30 mM KCl-induced [3H] ACh release. In the presence of β-CCM (10-8M to 10-4M), the release of [3H] ACh by KCl was not affected, but that by CCK8 was significantly inhibited depending on the concentra-tions of β-CCM. These results show that the action of CCK8 to stimulate neurons in the myenteric plexus can be selectively antagonized by β-CCM.
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