血清および尿中メタネフリンの直接ラジオイムノアッセイ

概要

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A simple, direct and specific radioimmunoassay for serum and urinary metanephrine was developed, in which we used <SUP>125</SUP>I-Synephrine and specific antiserum generated in rabbits by injecting with metanephrine conjugated with bovine serum albumin as described by Grota and Brown. <BR>The sensitivity of the assay was 2 pg/tube. Intra-and inter-assay coefficients of variation were 2.6-7.8% and 5.0-9.6%, respectively. The recoveries of metanephrine added at two levels of 250 pg/ml and 500 pg/ml to ten serum samples and ten urine samples (hydrolyzed and diluted) were 96.7 and 108% in serum, and 94.8 and 103% in urine, respectively. <BR>Normal values of serum metanephrine were 40.3±25.8 pg/ml (mean±SD) from 20 normal subjects. Normal values of 24 hour urinary metanephrine excretion were 12.5±6.7 ug/day from 24 normal subjects. Serum metanephrine values for 7 patients with pheochromocytoma were 210-628 pg/ml. Urinary metanephrine values for 9 patients with pheochromocytoma 8.7-302 ug/day.