尿中アルドステロンの直接ラジオイムノアッセイ法

概要

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A simplified direct radioimmunoassay for urinary acid labile aldosterone was developed. One ml of urine was hydrolysed with 2ml of 0.2N HC1 at 30°C for 16hrs. One tenth ml of hydrolysed urine diluted 10 times with charcoal treated aldosterone-free calf serum was used for the radioimmunoassay. The radioimmunoassay was done with a specific antibody, <SUP>125</SUP>I-aldosterone, as the labeled antigen and polyethylene glycol for bound-free separation. There were excellent correlations between the present methods and other methods, i.e., i) a method using dichloromethane extraction before the assay as well as pre-extraction before hydrolysis and ii) a commercial kit using <SUP>3</SUP>H-aldosterone. The intra-assay coefficient of variation was 5.8%, and the inter-assay coefficient of variation was 9.5%. The normal value of urinary aldosterone excretion was 3.7 ± 2.5pg/day by the present method, and values of patients with primary aldosteronism were between 24 to 43μg/day.