各種妊娠性蛋白の妊娠初期および末期の絨毛組織内濃度に関する検討

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We examined the localization of five pregnancy-associated proteins such as pregnancy specific β1-glycoprotein (SP1), human chorionic gonadotropin (HCG), human placental lactogen (HPL), α2-PA-glycoprotein (SP3) and pregnancy-associated plasma protein-A (PAPP-A) in the placentae of early and term pregnancies by means of the PAP method in immunohistochemical technology. It was found that the degree of staining of these proteins did not reflect their concentration in serum at each gestational age. Therefore, the concentrations of these proteins in the solution extracted from the placenta were measured. 1) Placental tissues were fixed with a 10% neutral formaldehyde solution and these were embedded into paraffin blocks. These specimens were used for the PAP method. The localizations of five pregnancy-associated proteins from 10 placentae at the gestational age of 7 to 10 weeks were compared with those at the gestation age of 38 to 41 weeks from 7 patients. The staining degree of SP1 in free villi was pale in both early and term pregnancies. HCG was stained deeply in early pregnancy but pale at term pregnancy. HPL was stained deeply at both gestational ages. Horseradish peroxidase reaction products from SP1, HCG and HPL were located chiefly in the syncytiotrophoblast. SP3 was not stained on the placental tissue. PAPP-A was stained to a greater extent in the cytotrophoblast in early pregnancy but pale in the syncytiotrophoblast at term pregnancy. 2) The concentrations of SP1, HCG, HPL, SP3 and PAPP-A were measured in the placental tissues in both early and term pregnancies. Placental tissues were obtained from 24 normal pregnancy patients aborted artificially at 8 to 11 weeks gestation and from 28 patients terminated by normal deliveries at 38 to 40 weeks gestation. The tissue was homogenated with 3 volumes of 1/2 PBS (0.005M phosphate-buffered 0.07M sodium chloride, pH 7.4). The supernatant was removed after centrifugation and stored at -20°C until assayed. The concentration of SP1 was measured by single radial immunodiffusion. That of HCG was assayed by a directed Latex aggulegation test (Gestate Slide Eiken). The concentrations of HPL, SP3 and PAPP-A were quantified by rocket immunoelectrophoresis. No significant difference in SP1 levels was shown between early and term pregnancies, and the SP1 level was 4.3 ± 1.3 (mean ± 1 S.D.) mg/dl at term pregnancy. The HCG level was 1,100 ± 300 IU/ml in early pregnancy and at least 20-fold higher than that at term pregnancy. The HPL level was 79.1 ± 27.6 mg/dl at term pregnancy. This value was much higher than that of early pregnancy. The SP3 level was hardly detected in both early and term pregnancies. PAPP-A was not detected in early pregnancy, but this concentration increased up to about 50% of the serum level at term pregnancy. The concentration of each protein in placenta was compared with the serum standard level determined in Mie University. At term pregnancy, the serum SP1 level was 4-fold greater than the placental SP1 level. The HCG level was not significantly different between serum and placenta. The placental HPL level was about 160-fold greater than the serum HPL level at term pregnancies. The serum SP3 level was 35.6 ± 17.4 mg/dl, but the placental SP3 level was very low. Mean placental weight, after umbilical cords and membranes were removed, was 373 ± 97 gr at full term delivery. One term placenta contained 15.7 ± 4.5 mg of SP1, 21,000 ± 11,000 IU of HCG and 317 ± 151 mg of HPL. These results suggest that the function of SP1 is biologically unique, different from other pregnancy-associated proteins, and SP1 may be secreted positively in the blood flow.
日本内分泌学会の論文

各種妊娠性蛋白の妊娠初期および末期の絨毛組織内濃度に関する検討

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