Rapid-Air-Dry Papanicolaou Stain in Canine and Feline Tumor Cytology: A Quantitative Comparison with the Giemsa Stain

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概要

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• The Papanicolaou stain is a gold-standard staining method for tumor diagnosis in human cytology. However, it has not been used routinely in veterinary cytology, because of its complicated multistep procedure and requirement for wet fixation. Currently, a rapid Papanicolaou stain using air-dried smears is utilized in human cytology, but usefulness of this rapid-air-dry Papanicolaou (RAD-Pap) stain in the veterinary field has not been fully evaluated. The purpose of this study was to evaluate the usefulness of the RAD-Pap stain by using quantitative analysis. Air-dried impression smears were collected from tumor specimens and stained with RAD-Pap and Giemsa. Twelve parameters representing the criteria of malignancy were quantitated, and characteristics of the RAD-Pap were evaluated statistically. The RAD-Pap stain could be applied to all the smears, and images of nucleoli and chromatin patterns were clear and detailed. In quantitative analysis with the RAD-Pap stain, but not with the Giemsa stain, dispersion of nucleolus size and dispersion of nucleolus/nucleus ratio in malignant tumors were significantly higher than those in benign tumors. These findings demonstrated that the RAD-Pap stain was useful for obtaining detailed nuclear information, and the ability to differentiate benignity and malignancy by nucleolus findings was a principal advantage of this stain. This RAD-Pap stain could be routinely used as a supportive staining method in veterinary diagnostic cytology.

著者

• Yamato Osamu

  Laboratory of Veterinary Internal Medicine, , Department of Veterinary Clinical Sciences, Graduate S

• Yabuki Akira

  Laboratory Of Veterinary Anatomy Department Of Veterinary Medicine Faculty Of Agriculture Kagoshima

• MIYOSHI Noriaki

  Laboratory of Pathology, Department of Veterinary Medicine, Kagoshima University

• YABUKI Akira

  Laboratory of Veterinary Clinical Pathology, Department of Veterinary Medicine, Kagoshima University

• MIYOSHI Noriaki

  Laboratory of Veterinary Pathology, Department of Veterinary Medicine, Kagoshima University

• SAWA Mariko

  Laboratory of Veterinary Clinical Pathology, Department of Veterinary Medicine, Kagoshima University

• ARAI Kou

  Kagoshima University Veterinary Teaching Hospital, Kagoshima University

• YAMATO Osamu

  Laboratory of Veterinary Clinical Pathology, Department of Veterinary Medicine, Kagoshima University

• YAMATO Osamu

  Laboratory of Clinical Pathology, Department of Veterinary Medicine, Kagoshima University

• YABUKI Akira

  Laboratory of Clinical Pathology, Department of Veterinary Medicine, Kagoshima University

• MIYOSHI Noriaki

  Laboratory of Histopathology, Joint Faculty of Veterinary Medicine, Kagoshima University

• YABUKI Akira

  Laboratory of Clinical Pathology, Department of Veterinary Medicine, Joint Faculty of Veterinary Medicine, Kagoshima University

• YAMATO Osamu

  Laboratory of Clinical Pathology, Department of Veterinary Clinical Sciences, Faculty of Agriculture, Kagoshima University

• SAWA Mariko

  Laboratory of Veterinary Clinical Pathology, Department of Veterinary Medicine, Kagoshima University, Kagoshima 890–0065, Japan

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