Pasteurella piscicidaで免疫したハマチの凝集抗体価と血清タンパクの変化

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Development of vaccine against pseudotuberculosis, caued by Pasteurella piscicida, in cultured yellowtail. Seriola quinquraiata, has been attempted, yet few studies of humoral aspect of the immune response in yellowtail have been reported. The present study was desiged to obtain some informations about agglutinating antibody titers and serum protein changes of cultured yellowtail immunized by intraperitoneal injection with formalin-killed P. piscicida cells in FREVND's complete adjuvant. Agglutinating antibody titers in immunized fish sera increased ranging from 1:256 to 1:2, 048 five weeks after immunization. Serum protein changes after immunization were observed by electrophoretic analyses; cellulose acetate electrophoresis, polyacrylamied gel disc electrophoreses and agar gel immunoelectrophoresis. Both immunized and control fish sera were separated into four fractions by gel filtration on Sephadex G-200 and agglutinating antibody activity was found in the first fraction of immunized one. This fraction included the protein, more than approximately 800, 000 inmolecular weight, the changes of which wre observed in number and density of precipitin lines formed in α-β _globulin region in agar gel immunoelectrophoresis after immunization. It is concluded that production of agglutinating antibody results in serum protein changes, observed in agar gel immunelectrophoresis, after parenteral immunization.
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