養殖アユのビブリオ病に対する噴霧ワクチンの研究-I : ワクチンのベントナイト添加とpH調整の必要性

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Differences in efficacy among bentonite-added and/or pH-adjusted vaccines for spray vaccination against vibriosis in cultured ayu, Plecoglossus altivelis were investigated. Vibrio anguillarum, the etiological agent of vibriosis in ayu, was incubated in nutrient broth with shaking for 96 hat 25°C. Then the culture was inactivated with 0.5% formalin for 24h at 20°C. Five kinds of vaccine used in this study were GOULDS vaccine, bentonite-added vaccine, pH-adjusted vaccine, formalin-killed vaccine and cell-free vaccine. Experimental groups of fish placed in dip net were sprayed with these vaccines at a pressure of 5kg/cm2 for 5-7 seconds by the sand blasting gun positioned 20-25cm from fish. The artificial water-borne challenge with virulent V. anguillarum was carried out two weeks after vaccination. All except the cell-free vaccine were highly effective. From the experimental result the formalin-killed vaccine was also effective as well as GOULDS vaccine; it can be concluded that it is not necessary to add bentonite or to adjust pH of spray vaccine for the control of vibriosis in cultured ayu. All the fishes sprayed with cell-free vaccine died two days after that of the control. This might indicate that cell-free vaccine contains the soluble antigens, which play a part in the protection of the pathogenic infection to some extent. Increase in agglutinating antibody titers of fish sera was observed in neither the immunized fish nor the control.
公益社団法人日本水産学会の論文

養殖アユのビブリオ病に対する噴霧ワクチンの研究-I : ワクチンのベントナイト添加とpH調整の必要性

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