Streptococcus sp.に対するブリマクロファ-ジの食菌作用に及ぼす抗体および補体のオプソニン効果

概要

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In order to know the defense mechanism of yellowtail Seriola quinqueradiata against Streptococcus sp, infection, phagocytic response of glass adherent macrophage-like cells isolated from normal fish was examined after one hour incubation at 25°C. Phagocytosis was observed in Giemsa stained smears under light microscope. Opsonic effects of specific antibody and com-plement were also investigated. Specific antibody increased the phagocytic activity and phagocytic index significantly. After addition of complement neither phagocytic activity nor phagocytic index changed significantly. In presence of specific antibody and complement, phagocytic activity and phagocytic index were significantly higher than in the control, and higher than in the previous tests. These results suggest that activation of the classical pathway of yellowtail complement by antigen-antibody reaction is an important function in opsonization of particulate antigen.