新奇コイ血漿フィブロネクチンの精製と性質〔英文〕

概要

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A novel carp plasma fibronectin, together with a typical plasma fibronectin reported previously (designated as carp plasma Type I fibronectin, CpFN-I, in this paper), was isolated by sequential column chromatographies on thermally denatured carp skin Type I collagen (carp gelatin)-Se-pharose, arginine-Sepharose and Sepharose CL-6B. This plasma protein could be separated from CpFN-I, because of its low affinity for the carp gelatin column. Judging from sodium dodecyl sulfate polyacrylamide gel electrophoresis in reducing and unreducing conditions, this protein preparation contained a single 205 kDa component together with a negligible amount of other related components. The protein resemble CpFN-I and bovine plasma fibronectin in amino acid and carbohydrate compositions, and was very similar but not identical to CpFN-I in peptide maps obtained by digestion with several proteases. However, it differed clearly in molecular size from CpFN-I. This plasma protein also showed immunological cross-reactivity to antiserum against CpFN-I. These results suggest that this protein is a novel plasma fibronectin, which is closely related to but distinct from CpFN-I. Thus, we tentatively named this novel glycoprotein carp plasma Type II fibronectin (CpFN-II).
日本水産學會の論文