改良加水分解条件によるアルギン酸M/G比の測定〔英文〕

概要

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Decomposition of alginate under the hydrolysis procedure with sulfuric acid was evaluated by determining the recovery of monouronates produced and its depolymerization extent. The first three hour hydrolysis with 80% sulfuric acid at 30°C, followed by the two hour hydrolysis with 2 N sulfuric acid at 100°C, resulted in complete hydrolysis with the highest recovery in the conditions examined. Under these conditions, the recovery of authentic uronates was 80.9% for D-man-nuronic acid and 62.8% for L-guluronic acid. Prolonged treatment resulted in a lower recovery of monouronates, and showed indications of an increase in the degradation of monouronates. The degradation products showing reducing power, were detectable with the phenol-sulfuric acid method but undetectable with the carbazole-sulfuric acid method. D-Mannuronic acid to L-guluronic acid ratios (M/G ratio) of nine species of alginates determined by the improved procedure were compared to those determined by the method of Haug and Larsen. The values of M/G ratio obtained by both methods were nearly equal in six species and were slightly different among three specíes. The modified method, however, showed a 9∼17% higher recovery of monouronates than the method of Haug and Larsen. In addition, the time for the completion of the analysis process was shortened by at least 1/5.
日本水産學會の論文