タツナミガイ多糖分解酵素の分布〔英文〕

概要

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The gastric teeth and midgut gland of a marine gastropod Dolabella auricularia were investigated with respect to the distribution of polysaccharide saccharification activities and glycosidase activities. Crude enzymes from both organs were obtained by extraction with a buffer (pH 7.5) containing 0.5M NaCl and their activities were measured for twelve kinds of polysaccharides and seven kinds of PNP-glycosides. The total enzyme activities (Units/organ) for saccharification of soluble starch, CM-cellulose, alginic acid, β-1, 4-mannan, and β-1, 3-glucan were found to be relatively high in both organs. The specific activities (Units/mg protein) of these enzymes in the gastric teeth exhibited two-to eightfold of those in the midgut gland. As for glycosidases, their activities were mainly distributed in the midgut gland, when compared to the gastric teeth in which only β-glucosidase, β-mannosidase, and α-glucosidase activities were detected.
日本水産學會の論文