STUDIES ON THE UNCLASSIFIED MYCOBACTERIA:II. An Interesting Strain of the Unclassified Mycobacteria (Terai Strain)

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A strain of unclassified mycobacteria was isolated from a resected pulmonary lesion as: reported previously. In the present experiment, the bacteria were inoculated to ddN-mice through tail vein and observations were made for 6 to 8 weeks under various experimental conditions. The results were as follows. (1) Single inoculation with 0.1mg Following the inoculation through tail vein, animals were sacrificed at 2 weeks, 4 weeks and 8 weeks, 3mice at each time. The autopsy findings were compared with those of the mice inoculated with Kurono strain, a virulent strain of human type tubercle bacilli, and treated in the same way as the experimental group. Consequently, the spleen and kidney were observed to increase in size with the lapse of time. There appeared no tubercles macroscopically in any of the organs but the microscopic examinations disclosed tubercles in spleen, liver, lung and kidney. They were marked in spleen and liver but less in lung and kidney. In contrast, the mice treated with Kurono strain showed macroscopic tubercles in lung already after two weeks and increased in number as time progressed. Colony counts were made on each organ, revealing many living bacteria in every organ, even 8 weeks after the inoculation. They were more in number in spleen and liver than in lung. In the mice treated with Kurono strain, contrarily colony growth was most obvious in lung. Colony counts tended to become less, though with slight degree, as time progressed after the inoculation in spleen, liver and lung. This tendency was similar in the control group with Kurono strain. In kidney, however, colony counts seemed still increasing, even 8 weeks after the inoculation. (2) Single inoculation with 1.0mg. Mice were inoculated with 1.0mg of the bacteria after a month's control observation and were sacrificed 2 weeks, 4 weeks and 8 weeks later, 3 animals at each time. The results were similar to the ones in (1) with slight difference. At 2 weeks and 4 weeks after the inoculation, spleens were twice heavier, than those of the group inoculated with 0.1mg Colonies and microscopic tubercles were also generally more in number than in the group inoculated with 0.1mg. (3) Multiple inoculation with 0.1mg This experiment was done parallel to (1). Every mouse was inoculated with 0.1mg of the bacteria. Two weeks later the second inoculation was given to the mice except for 3 that were sacrificed at the end of 2 weeks. These procedures were repeated in the same way, 3 mice being sacrificed and the rest being submitted to the next inoculation with 0.1mg of bacteria, until the fourth inoculation. The results were approximately the same as in (1), spleens being markedly heavier, colony counts and numbers of the tubercles being numerous in this group than in (1), as observed in (2). (4) Inoculation with the Terai strain into the mice which were infected with tuberculosis and treated with chemotherapy experimentally.
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STUDIES ON THE UNCLASSIFIED MYCOBACTERIA:II. An Interesting Strain of the Unclassified Mycobacteria (Terai Strain)

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