Examination for Pyrazinamide-resistant Tubercle Bacilli:Fifth report: Statistical observation of the results using 1% Ogawa eggmedia pH 5.5 for examination
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Sawai previously reported that 1% Ogawa egg-media pH 5.5 can be used to examine pyrazinamide resistant tubercle bacilli. The authors have utilized this method for routine examina tion and then observed statistically the results of 220 cases, examined the growth of tubercle bacilli, approval result of medium, relationship between pyrazinamide dose and resistant rate.<BR>1) When 1% Ogawa egg-mdia ph 5.5 and 3 Ogawa egg-media are compared the positiverate of growth of tubercle bacilli, colony number, period until colonies develop are inferior in 1% Ogawa egg-media pH 5.5 as compared to those in 3% Ogawa egg-media.<BR>2) When the patient with INH resistant bacilli is given pyrazinamide and INH, tubercule bacilli from a patient with INH resistant bacilli easily becomes pyrazinamide resistant than a patient with INH sensitive bacilli.<BR>3) The pH of 1 % Ogawa egg-media pH 5.5 brings about slight changes even by the same turing process, and on this medium pyrazina mide sensitive tubercle bacilli sometimes grow over 10<I>g/cc</I> while only a few colonies are found on the control media.<BR>For this reason, when preparing this medium it is always necessary to examine this with pyrazinamide sensitive tubercle bacilli, and if the results of only those passing this examination are taken, this medium can be used for routine test.
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- Examination for Pyrazinamide-resistant Tubercle Bacilli:Fifth report: Statistical observation of the results using 1% Ogawa eggmedia pH 5.5 for examination
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