人型結核菌と他の抗酸菌との鑑別方法としてのナイアシンテスト

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In a previous experiment niacin test on the cultures of tubercle bacilli grown on Ogawa egg slant with an airtight rubber stopper revealed that approximately 28% of the test strains were negative. Such a high incidence of the negative strains was considered to be due to an insufficient amount of the growth used. The objective of this paper is, firstly, to confirm the above assumption using a large number of strains and, secondly, to answer the question whether or not the resistance to the primary antituberculous drugs brings about the negative reaction in the bacilli.<BR>472 strains isolated from treated or non-treated patients were submitted to the routine drugsusceptibility test. Each of the control cultures was transferred by loop onto four Ogawa egg slants which were then divided into equal two; the first two tubes were sealed with an airtight rubber stopper while the other with a cork stopper. After 4 weeks of incubation at 37°C, each one tube exhibiting more abundant growth was directly subjected to the niacin test according to BrCN-benzidine method. The results were summarized in Table 1. It is seen that the niacin positive rate in the series with cork stopper was 98.7% while the rate of the series with airtight rubber stopper was 89.4%. Moreover, the difference in degree of the positive reaction was observed between the two series. Namely, most of the cultures with cork stopper, 93.6%, revealed strongly or moderately positive reaction, whereas in the series with rubber stopper about 80% of the cultures were found to be only weakly or doubtfully positive. Tables 1 and 2 show that the amont of growth as well as the number of colonies developed was superior in the series of cork stopper. It will be suggested that in order to perform direct niacin test the culture tube must be sealed with such a stopper or cap allowing free-entry of air.
日本結核病学会の論文

人型結核菌と他の抗酸菌との鑑別方法としてのナイアシンテスト

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