ナイアシン-テスト試験紙法の改良

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Several modified methods of the niacin test have been made with a reagent impregnated paper strip. As it was thought that various methods have to be improved in their stability, sensitivity and feasibility, the authors prepared a new niacin test paper and compared with the routine niacin test. I) Method to prepare the test paper Following reagents were used; 50% acqueous chloramine T, 60% KSCN in 8% citricacid and 10% para-aminosalicylic acid (PAS) in 95% ethanol. Filter paper was cut into 50mm by 3mm strips. Acryl sheet was cut into the same size. One set of our test paper needs two paper strips and one acryl sheet. Using a capillary pipette, one drop of 60% KSCN in 8% citric acid and 3 drops of 50% acqueous chloramin T were added to the strip (a). Two reagents were not allowed to diffuse into one another on the paper strip. One drop of 10% PAS in 95% ethanol was added to the strip (b). Acryl sheet was placed between the two paper strips. Strip (b) is the front and strip (a) is the back of the test paper. They are covered with the vinyl sheets. The front of the cover is colorless transparent vinyl, and the back is the white one. The vinyl cover was sealed except its foot (Fig. 2). This test paper is kept in a brown, transparent plastic bottle with silicagel and left at room temperature. II) Method of experiments 1.5ml of sterile distilled water was added into the 1% Ogawas egg media with well grown colony, and laid down on the slope for 5 minites to gain the extract. 0.2ml of the extract was divided into a test tube for the routine niacin test. The test paper was placed in a tube of 1% Ogawas egg media for 15 minites. The foot of the test paper is set in the extract. The development of a orange color at the part of the PAS is positive, and the absence of this color is negative. (Fig. 1) III) Results 1) The test paper method and the routine niacin test showed nearly the same results, and the test paper presented positive reaction to 2mg/ml niacin. (Table 1) 2) The both methods presented nearly equal reactions in 158 strains isolated from sputa of pulmonary tuberculous patients. (Table 2) 3) The effect of the quantity of the sterile distilled water added into 1% Ogawas egg media to gain the niacin extract was examined with 11 strains isolated from sputa of pulmonary tuberculous patients. 10-2mg of each strain was inoculated and cultured. The less the quantity of the water, the stronger the reaction. (Table 3) In conclusion, the merits of our test paper were summarized as follows; (a) There is no fear of gas poisoning. (b) This method does not need a procedure to divide the extract containing numerous bacilli. (c) A paper is easily preserved.
日本結核病学会の論文

ナイアシン-テスト試験紙法の改良

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