NIACIN TEST FOR DIFFERENTIATION OF MYCOBACTERIUM TUBERCULOSIS VAR. HOMINIS FROM OTHER ACID-FAST BACILLI:VI. A few notes on evaluation of procedures
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We have performed niacin test by using cyanogen bromide benzidine method on many strains of human tubercle bacilli with various number of colonies and varying degrees of, growth, which were isolated on 3% Ogawa media from sputa or laryngeal swabs. And we investigated whether or not solvent for the extraction had to be heated and whether or not the false positive results due to the insufficient amount of bacilli could be reduced to zero by prolonging the extraction time or by microtechnique devised by M. S. Tarshis. The following were results obtained.<BR>1) Solvents for extraction: Comparative examinations were carried out on each of 56 strains by using hot and cold distilled water and on each of 65 strains by using hot and colphysiologic saline, which revealed no remarkable differences in the positive rates.<BR>2) Extraction time: Each of 47 strains was extracted with cold physiologic saline for-10, 30 and 60 minutes and the results of these three groups were compared, revealing similar positive rates. Namely, for the practical purpose, no more than 10 minutes was required for the extraction.<BR>3) Microtechnique: Conventional-and micro-techniques were applied to each of 354 strains and the results compared, which revealed no significant difference. The positive rate could not be raised with microtechnique.
- 一般社団法人 日本結核病学会の論文
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