Niacin Test for Differentiation of Mycobacterium Tuberculosis Var. Hominis from Other Acid Fast Bacilli.:IV. Difference in Results Obtained with Various Media.
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Satisfactory results were obtained when niacin test was performed on human tubercle bacilli isolated or successively transferred on the egg culture media, as reported previously. However, egg medium is not the sole medium either for the isolation of tubercle bacilli or for the sensitivity test for chemotherapeutic agents, agar media also being used for such purposes, although smaller in number. Therefore, experiments were made on whether or not niacin tests can be conducted with tubercle bacilli cultured on agar media as satisfactorily as with those on egg media.<BR>O.1 ml. of sputum treated with 4 % NaOH was inoculated on each of 3 % Ogawa egg medium, modification III of Kirchner's agar medium containing serum and B II medium containing whole blood, while O.1 ml. of neutralized sputum pretreated with 4 % NaOH was inoculated on each of 1 % Ogawa egg medium, Kirchner's agar medium containing serum, B I medium containing whole blood and Tarshis'medium. By using 366 strains of tubercle bacilli with various numbers of and various sizes of colonies grown for one to two months of observation periods, 1, 101 niacin tests were carried out by means of cyanogen bromide-aniline method (anilin BrCN method)modified by Runyon and 1, 154 tests by means of cyanogen bromide-benzidine method (benzidine BrCN) in order to see the effects of malachite green and hemoglobin intermixed with the extract of tubercle bacilli on results of the color reaction, that is, how often these substances rendered the evaluation of the test results difficult or impossible. At the same time, the incidence of positive tests obtained from tubercle bacilli grown on the media containing serum or blood was investigated according to the number and the size of colonies.
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