結核菌と非定型抗酸菌の混在している菌株よりの両菌の分離の経験 (抗酸菌の分離培養法の改良-5,6-)

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A certain: Minowa, a 30-year-old company employee, was admitted to our hospital with diagnosis of the relapse of pulmonary tuberculosis in October 1965. Acid-fast organisms w ere isolated twice from his sputum; one rough colony was obtained in the above month w hich proved niacin-positive and smooth orange-colored fast-growing mycobacteria, 33 colonie s per tube, were isolated in March of the next year. Drug sensitivity test shown in the Table 1 revealed that some culture of the latter strain 'contained a few rough colonies among smooth ones. All of the six cultures, therefore, were submitted to examination for purity. On preliminary subcultivation (Table 2) it was found that out of six cultures at 37°C two (# 5 and # 6) contained rough colonies and four (# 1, 4, 5 and #6) were niacin-positive. Further, the method described in the preceding paper wa#s applied to these cultures: a 1m g/ml aqueous suspension was prepared from each of the grow th 'of cultures. From these suspensions serial 10-fold dilutions were made up using distilled water, 4% NaOH solution, and 4% H2SO4 solution, respectively. Each aqueous dilution was inoculated into four 1% Ogawa egg slants two of which were incubated at 37°C and the other two at: 22°C. The alkaline and acidic dilutions were inoculated into 3% Ogawa egg slants and 3% Na2HPO4 egg slants, respectively. They were incubated at 37°C. After one-month incubation the resulting growth was read on the assumptions that rough colony was tubercle b acilli and smooth orange-colored one as atypical mycobacteria. The number of colonies per tube of the two types organisms was recorded in Table 3. It will be seen that all substrains except 5# produced a few rough colonies in slants inoculated with the aqueous or alkaline dilution. On the other hand, smooth pigmented colonies were obtained on most of the slants at either temperature. For identification colonial subcultures on Ogawa egg medium were made of these rough and smooth colonies differentiated as above. The growth characteristics and niacin test shown in Table 4 indicate that all subcultures of the rough colony except one are the pure culture of the tubercle bacilli and all of the smooth colony are the same atypical mycobacteria as read before. One subculture which was derived from the aqueous suspension of the rough colonies of strain Minowa # 6 was found to be a mixed population of tubercle bacilli and atypical mycobacteria. Drug sensitivity test was carried out on these purified cultures. Table 5 shows the result of the test being compared with that of the test on the tubercle bacilli which were isolated from the lung lesion of the patient. It will be concluded that the method applied above is useful for such cases.
一般社団法人日本結核病学会の論文

結核菌と非定型抗酸菌の混在している菌株よりの両菌の分離の経験 (抗酸菌の分離培養法の改良-5,6-)

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