ヒトHLAクラスII抗原に対する単クローン抗体 : I 血清学的反応パターンの解析

概要

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Monoclonal antibody Sa3 was obtained by fusion of the spleen cells from the BALB/c mice immunized with Epstein-Barr virus (EBV)-transformed human B lymphoblastoid cell line, EBVSA (HLA-A24; B7; C-; DR1; DQ1; DP4) with mouse myeloma NS-1 cells. In the microcytotoxicity test, this antibody reacted with EBV-transformed B lymphoblastoid cell lines, Burkitts lymphomas, Pre-B leukemia and peripheral B cells, but not with T cell leukemias, Myelomonocytes, human spleen cells, human bone marrow cells or peripheral T cells. By SDS gel electrophoresis, the monoclonal antibody Sa3 detected on the HLA class II molecules was found to consist of two subunits of α chain (31-34k mol. wt.) and β chain (28-29k mol. wt.). These results suggest that monoclonal antibody Sa3 reacts with human HLA class II antigens.
日本細菌学会の論文