ROLE OF VITAMIN B12 AND ENZYMES RELATED TO METHYLMALONYL-CoA MUTASE IN A METHANOL-UTILIZING BACTERIUM, PROTAMINOBACTER RUBER

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A methanol-utilizing bacterium, Protaminobacter Tuber, required cobalt ion or vitamin B12 as its growth factor, which could be replaced by succinate among various additions to the cobalt-deficient medium. The presence of adenosylcobalamin (adenosyl-B12)-dependent methylmalonyl-coenzyme A (CoA) mutase was demonstrated in the cellfree extracts of P. ruber. The specific activity of this mutase was not only fairly high in comparison with that reported with other organisms but also detected at a similar level throughout the cultivation period. The cell-free extracts of P. ruber grown on non-C1 compounds as a sole carbon and energy source also had methylmalonyl-CoA mutase activity. Furthermore, the extracts of this microorganism catalyzed the reactions from propionyl-CoA to succinyl-CoA and from α-ketoglutarate to a-hydroxyglutarate.
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