CHEMICAL STUDIES ON VITAMIN B12 AND ITS RELATED COMPOUNDS:VII. PAPER-ELECTROPHORETIC EXAMINATION ON COBAMIDES IN PROPIONIBACTERIUM SHERMANII CELLS CULTIVATED IN THE PRESENCE OF HYDROXOCOBALAMIN OR CYANOCOBALAMIN

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When Propionibacterium shermanii is cultured in the presence of OH-form or CN-form of cobinamide-like compound (X factor) together with 5, 6-dimethylbenzi-midaxole or in the presence of OH-B12 or CN-B12 alone, a large quantity of E. coli-active substance accumulated in the cells. In order to know the forms and the amounts of the active substance, an alcoholic extract of the cells was prepared and subjected to paper electrophoresis. The paper was cut into pieces and after extraction of each piece the extract was determined by the tube assay.At least 4 forms were found to be present. There was no great difference between OH- and CN-form, but the yield was greater after addition of cobamide than after addition of both cobinamide and 5, 6-dimethylbenzimidazole.
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