CHEMICAL STUDIES OF VITAMIN B12 AND ITS RELATED COMPOUNDS:X. NATURAL OCCURRENCE OF COBAMIDE COENZYME AND OTHER VITAMIN B12-ACTIVE SUBSTANCES IN THERMOPHILIC METHANE BACTERIA

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概要

• 論文の詳細を見る

• 1. Little has been known about the native forms of vitamin B12-active substances and the natural occurence of Barker's cobamide coenzyme in the cells of methane bacteria, since most of non-cyano type vitamin B12 were transformed to the cyano type by the extraction procedure using cyanide solution previously employed.The authors employed an extraction method using hot ethanol instead of the cyanide treatment in order to avoid the transformation of cyanide-labile vitamin B12 analogues.2. A purified extract was obtained by the usual phenol treatment of the alcohol extract. By paper ionophresis performed in 0.5N acetic acid, five zones active to E. coli No. 215 were observed: three of them (tentatively named P1-P3 in the order of the mobilities) moved toward the cathode, one (P4) stayed near the start and the fifth (P5) migrated toward the anode. On the other hand, disappearance of P1 and P2 and concomitant increase in the area of P4 were observed in the extract with cyanide.In the bioautogram using Ochromonas malhamensis which is known to have more specific responce to 5, 6-dimethylbenzimidazolyl cobamide than E. coli, the growth zones corresponding to P2 and P3 were not detected.3. The partial purificate was fractionated by DEAE-cellulese column chromatography. A fraction eluted with water contained the vitamin B12-active substance corresponding to the zone P1. The fraction exhibited the coenzyme activity in Abeles-Lee's propanediol-propionaldehyde conversion.4. From these results it seems appropriate to conclude as follows: P1 corresponds to DBCC; P2, non-cyano type B12 analogue lacking 5, 6-dimethylbenzimidazolyl cobamide structure; P3, pseudocobalamin; P4, cyanocobalamin and P5, an unidentified B12 analogue with anionic character, the paper ionophoretic behavior of which is analogous to that of vitamin B12 coenzyme M of Takeyama and Buchanan.

• 日本ビタミン学会の論文

著者

• 清水 祥一

  Department fo Food Science and Technology, Faculty of Agriculture, Nagoya University

• 酒沢 千嘉弘

  Department of Applied Chemistry, Himeji Technical College

• 高橋 敏夫

  Department of Industrial Chemistry, Faculty of Engineering, Kyoto University

• 福井 三郎

  Department of Industrial Chemistry Faculty of Engineering, Kyoto University

• 加藤 忠克

  Department of Idustrial Chemistry, Faculty of Engineering, Kyoto University

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