Histochemical Observation and Cellular Distribution of Ascorbic Acid in Persimmon Leaves
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概要
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This study was carried out to resolve the discrepancy of data for the proportion of ascorbic acid and dehydroascorbic acid in persimmon leaves at the final stage of the season and to clarify their cellular distributions using histochemical and biochemical techniques. Fresh persimmon leaves were collected and used on July 31, September 5 and October 7, 1996. Ascorbic acid and dehydroascorbic acid in sub-cellular fractions were determined by the HPLC method that was found to be the most reliable for separation. The percent of dehydroascorbic acid in the total leaves was found to be almost constant (between 32 and 37%) in all preparations tested. In all preparations, more than 90% of the ascorbic acid and dehydroascorbic acid was found in the soluble fraction. The histochemical detection of ascorbic acid and an electron micrograph of persimmon leaf cells showed that the reactive color, after the reduction of silver nitrate under acidic conditions, in the leaves of all three preparations was mainly found on the face side of columned-type palisade parenchyma cells where chloroplasts were not rich and large vacuoles were seen. On the inner side of the palisade parenchyma cells where chloroplasts were the richest, only weak color development was observed. This study demonstrates that the percent of dehydroascorbic acid in persimmon leaves did not exceed 40% at least until October 7. It also shows that in persimmon leaf cells, ascorbic acid is mainly localized in the cytosol of palisade parenchyma tissue cells where large vacuoles are seen.
- 財団法人 学会誌刊行センターの論文
著者
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INNAMI Satoshi
Department of Applied Biology and Chemistry, Faculty of Applied Bioscience, Tokyo University of Agri
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MAEKAWA Akio
Department of Applied Biology and Chemistry, Faculty of Applied Bioscience, Tokyo University of Agri
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Hara Takayuki
Department Of Biochemistry Graduate School Of Health And Nutrition Sciences Nakamura Gakuen Universi
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Fujita Mamoru
Department Of Anatomy Kagawa Medical School
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Tadokoro Tadahiro
Department Of Applied Biology And Chemistry Tokyo University Of Agriculture
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KUSUNOKI Kikue
Department of Food and Nutrition, Nakamura Gakuen University
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MINARI Yoshimi
Department of Food and Nutrition, Nakamura Gakuen University
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TADOKORO Tadahiro
Department of Agricultural Chemistry, Tokyo University of Agriculture
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MAEKAWA Akio
Department of Agricultural Chemistry, Tokyo University of Agriculture
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HARA Takayuki
Department of Food and Nutrition, Nakamura Gakuen University
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FUJITA Mamoru
Department of Food and Nutrition, Nakamura Gakuen University
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