GLYCYLPROLINE DIPEPTIDYL AMINOPEPTIDASE ACTIVITY IN THE SERA FROM PATIENTS WITH HEPATOBILIARY DISEASES

概要

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An automated method is proposed for the assay of glycylproline dipeptidyl amino-peptidase (GPDA) activity in the serum by Rotochem ha centrifugal fast analyzer. The method has reduced technical involvement and offered considerable advantages in reproducibility and linearity. Good correlation was also obtained between the manual and automated methods. We also examined the activities of GPDA in sera from 256 patients with hepatobilliary diseases including 69 cases of hepatic cancer and from 100 healthy controls to investigate the diagnostic value of this enzyme. A highly significant elevation of the enzyme activity, more than 200 I.U./L, was found in 36% of sera from patients with hepatic cancer, but all other hepatobiliary diseases showed the enzyme activities less than 200 I.U./L in the sera with few exceptions.These results indicate that the assay of serum GPDA activity is useful for the differential diagnosis of hepatic cancer from other hepatobiliary diseases. The fact that no correlation was observed between the serum GPDA activities and α-fetoprotein levels may increase the clinical value of this enzyme for the early diagnosis of hepatic cancer.
財団法人日本消化器病学会の論文