ニトロフラン誘導体の食品防腐剤としての研究-XV. : 細菌芽胞の発芽増殖に及ぼすフリルフラマイドの影響について,顕微鏡観察による検討

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The effect of furylfuramide (FF) on germination and outgrowth of two bacterial spores was studied in a slide culture, in order to define the point at which FF acts to prevent the developmental process, from a morphological point of view. Concentrations of FF higher than minimal inhibitory concentration (MIC) did not interfere with the reduction of refractility. However, the developmental process stopped there, and the germinated spores eventually lysed before elongation occurred, shedding the empty spore coats. At less than MIC, many distorted cells appeared and gradually lysed. The bacteriostatic action of FF was shown as a result of the prolongation of their outgrowth. FF acted to arrest the development of bacterial spores not at germination, but after the stage from “swelling” to “emergence”. A discrepancy between the two organisms existed in that many refractile spores of B. coagulans, isolated from spoiled fish sausage, still remained on the culture after 35 hours at 37°C, but not in the case of B. subtilis.
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