脂肪酸アミドのガスクロマト法による分析法

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In the previous paper, the presence of fatty acid amides (RCONH2) in the visceral lipid of fin whale has been demonstrated. In this paper, the application of gas chromatography to the analysis of RCONH2 is discussed. On the SE-30 column, RCONH2 were easily detected by the direct injection of RCONH2. However the unsaturated RCONH2 did not get separated from saturated ones. On the other hand, on diethylene glycol succinate polyester column, RCONH2, prepared from visceral lipid of fin whale, got separated into many symmetrical peaks (Fig. 1). They were identified by using the relationship between the logarithm of retention time and carbon number (Fig. 2). Moreover, RCONH2 composition obtained by the direct injection method was compared with that obtained as fatty acid methyl esters (Table 1), and they were found to be practically identical to each other. Thus, the results reveal that this gas chromatographic method is not only convenient but also accurate for RCONH2 assay.
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