クジラ臓油中の脂肪酸アミドの分離および同定

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It was previously shown in our laboratory that certain lipids in visceral lipid of fin whale (Balaenoptera physalus), which were eluted chromatographically with methanol-chloroform after being eluted most of neutral lipid with ethyl ether, have infrared absorption at 5.96 and 6.28μ due to amide linkage. Judging from chemical tests, thin layer chromatography and infrared absorption spectroscopy, they seem not to be phospholipids, glycolipids nor sphingolipids. The amide lipids were separated by the procedure as shown in Fig. 1, and their components were analyzed after 2N-HCl hydrolysis in 80% methanol (8 hrs, 110°C). Ether soluble moiety of the lipids consisted of fatty acid methyl esters and small amounts of unmethylated fatty acids (Fig. 3). After remethylation with BF3 methanol, fatty acid methyl esters were analyzed by gas liquid chromatography. Twelve kinds (C12-C20) of fatty acids were detected and the composition was generally similar to that of original visceral lipid of fin whale (Table 1). On the other hand, water soluble moiety of the lipids was analyzed by paper chromatography and amino acid autoanalyzer, and ammonia and trace amounts of amino acids (0.07%/weight of the amide lipid) were detected. From a comparison between the amide lipids in visceral lipid and synthesized palmitoyl amide in infrared spectra and thin layer chromatogram (Fig. 2), it was concluded that the former are fatty acid amides.
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