自動酸化脂質中のプロスタグランジン様環構造を有する共役カルボニル類の定量〔英文〕

概要

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Conjugated carbonyls with a hydroxypentanone ring produced during autoxidation of methyl linolenate, methyl γ-linolenate, methyl arachidonate, ethyl eicosapentaenoate, methyl docosahexa-enoate and triglycerides of squid liver oil, were determined quantitatively using gas chromato-graphy-selected ion monitoring. A fraction containing the carbonyls was separated on a Sephadex LH-20 column connected with a high performance liquid chromatograph system. After NaBH4- reduction and subsequent trimethylsilylation, peak areas of a characteristic ion (m/z 167) of the carbonyls and a prominent ion (m/z 187) of the internal standard, 12-hydroxy methyl stearate, were measured quantitatively. Production of the carbonyls during autoxidation showed a good correlation with the degree of red coloration with glycine (A515nm) of the oxidized lipids. A maximum formation of the carbonyls at 30°C was estimated to be 0.04 to 0.11%. In addition to the carbonyls, much larger amounts of prostaglandin-like substances, precursors of the carbonyls, were observed in the relatively early stage of autoxidation.
日本水産學會の論文