Asparagine-linked sugar chains of plasma membrane glycoproteins from healthy and primary immunodeficient patients' B lymphoblastoid cell lines

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Asparagine-linked sugar chains of plasma membrane glycoproteins from healthy and primary immunodeficient patients' B lymphoblastoid cell lines (LCL) were investigated. LCL were, respectively, prepared from healthy individuals and two patients with common variable immunodeficiency, using Epstein-Barr virus. Each LCL had complement receptors, OKIal antigen and OKB antigen on the membrane. Crude membrane fractions were prepared by the hypotonic shock and the centrifugation. Analysis of the asparagine-linked sugar chains of plasma membrane glycoproteins was carried out by the chromatography and the sequential exogycosidase digestion. In results, the main oligosaccarides in asparagine-linked sugar chains of plasma membrane glycoproteins from healthy LCL were biantennary sugar chains with bisect N-acetyl-glucosamine (Gal2•GlcNAc2•Man3•GlcNAc•GlcNAc•Fuc•GIcNAcOT) and those from patients' LCL were the same sugar chains with above. There was no difference on the main sugar chains between two patients' LCL. On the other hand, the main oligosaccarides in asparagine-linked sugar chains of plasma membrane glycoproteins from T lymphoblastoid cell lines were biantennary sugar chains without bisect N-acetyl-glucosamine, according to our other report.
日本臨床免疫学会の論文