Asparagine-linked sugar chains of membrane glycoproteins from healthy and Wiskott-Aldrich syndrome patient's erythrocytes

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Asparagine-linked sugar chains of membrane glycoproteins from healthy and Wiskott-Aldrich syndrome patient's (WAS) erythrocytes were investigated. Membrane fractions were prepared by the hypotonic shock and the centrifugation. Analysis of the asparagine-linked sugar chains of glycoproteins was carried out by the chromatography and the sequential exoglycosidase digestion. In results, the main oligosaccaride in asparagine-linked sugar chains of the membrane of erythrocytes from healthy and WAS were biantennary sugar chain with bisect N-acetyl-glucosamine (Gal2•GlcNAc2•Man3•GlcNAc•GlcNAc•Fuc•GlcNAcOT) respectively. Moreover, there was biantennary sugar chain with fucose, but was not biantennary sugar chain without fucose, in both erythrocytes membrane.
日本臨床免疫学会の論文