ヒト胎児唾液腺分泌顆粒の微細形態と電顕組織化学的検討 : PAM染色とPA-TSC-SP染色

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The secretory granules of 18 and 24 week-old fetal parotid and submandibular glands were studied in the electron microscope, and by stainings with periodic acid-methenamine silver (PAM staining) and periodic acid-thiosemicarbazide-silver proteinate (PA-TSC-SP staining) to identify carbohydrate-containing macromolecules electron microscopically.The results were as follows;1) By means of the usual double staining, one kind of secretory granule was observed in 18 week-old fetal parotid and submandibular glands, and 24 week-old fetal parotid gland. They were serous type granules in electron microscopy. In 24 week-old fetal submandibular gland, two kinds of secretory cells were distinguished, which were serous and mucous types.2) 18 and 24 week-old fetal secretory granules were abundant in polysaccharides, and were entirely positive in PAM and PA-TSC-SP stainings.3) On two kinds of secretory granules of 24 week-old fetal submandibular gland, one was serous type granule and the other was mucous type granule in PA-TSC-SP staining. It was considered that the differentiation of the serous and the mucous cells had occured at 24 week-old fetal submandibular gland.