1. Observation of the Mucosa of Colon by Scanning Electron Microscope Report (1): Normal Mucosa of Colon

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The mucosa of colon obtained by biopsy with Colonofiberscope was subjected to the scanning-electronmicroscopical observation. The specimens were obtained from the normal mucosa of descending and sigmoid colon.<BR>Method :<BR>The specimens were washed with physiological saline solution, fixed with 2.5% glutalaldehyde with phosphate buffer, washed with chymotrypsin solution, postfixed with millonig solution and dehydrated with graded alcohol solution. After coated with carbon and gold they were observed by scanning electron microscope, JSM-U3.<BR>Results :<BR>The mucosal surface showed multiple crypts, with which the surface looked like a honey comb. The crypts were surrounded by epithelial cells showing a stratified formtion. The microvilli of epithelial cells were observed under×1000, ×3000 and ×6000 of magnification. But, they were still not as clearly seen as those of small intestine because of mucous plugs attached to the microvilli. No definite morphological difference was noted between the sigmoid colon and descending colon.
日本大腸肛門病学会の論文