緬羊の血清学的体質に関する研究-6-血液型抗原およびそれら抗体の免疫化学的性状について

概要

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In order to clarify the immunochemical properties of cellulor antigenic substances of Sh1, A and D antigens, and the corresponding antibodies produced by heteroimmunizations, the present experiments were carried out by various methods. The results obtained were as follows:1) It was found that the antigenic substances of Sh1, A and D antigens exist in the protein-like fraction of blood stroma by absorption test, heat-resistance test and adjuvant immunization test.2) The activities of antibodies disappeared by heat-treatment at 70°C for 30 minuts, but they were not destroyed by treatment of 2-mercaptoethanol.3) The activities of antibodies were observed in the second peak of the protein fractions by the sephadex gel filtration.4) The antibodies eluted from antigens-antibodies complex were detected in γ-globulin zone by immunoelectropnereis.It was proved that the antibodies in these antisera were IgG type globulin.
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