Distribution of Single Nucleotide Polymorphisms in the CXCR1 Gene and Association with Calf Diseases in Japanese Black Cattle

概要

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The chemokine (C-X-C) receptor 1 (CXCR1) expressed on the neutrophil surface interacts primarily with interleukine-8 (IL-8) and make an important role in immune response. Two interesting single nucleotide polymorphisms (SNPs), SNP CXCR1+777G>C and SNP CXCR1-1768T>A that showed the association with subclinical mastitis and milk quality in daily cattle, respectively, have been reported in the bovine CXCR1 gene. The aim of this study was to demonstrate the presence of the two SNPs in the CXCR1 gene of Japanese Black cattle, and studied the association between the SNPs and clinical diseases including intestinal and respiratory diseases in the calves. The genotyping of the SNPs in healthy Japanese Black cattle showed that the SNPs also present in Japanese Black cattle with the gene frequencies of 0.37 and 0.15 for the C-type allele in SNP CXCR1+777 and of the A-type allele in SNP CXCR1-1768, respectively. The statistically analysis on genotype distribution of the SNPs in bovine CXCR1 gene in healthy and clinical intestinal or respiratory diseased Japanese Black cattle indicated no significant association of the SNPs with clinical diseases in the calves. However, regression correlation of the A allele number of SNP CXCR1-1768 with white blood cell (WBC) and platelet counts was found in the disease group. It is possible that the SNP in the bovine CXCR1 gene play a role in modulating the hematological profile of WBC and platelet counts.